Advantages of automation in plasma sample preparation prior to HPLC/MS/MS quantification: application to the determination of cilazapril and cilazaprilat in a bioequivalence study.
J AOAC Int
; 94(3): 758-64, 2011.
Article
en En
| MEDLINE
| ID: mdl-21797003
ABSTRACT
An HPLC/MS/MS method characterized by complete automation and high throughput was developed for the determination of cilazapril and its active metabolite cilazaprilat in human plasma. All sample preparation and analysis steps were performed by using 2.2 mL 96 deep-well plates, while robotic liquid handling workstations were utilized for all liquid transfer steps, including liquid-liquid extraction. The whole procedure was very fast compared to a manual procedure with vials and no automation. The method also had a very short chromatographic run time of 1.5 min. Sample analysis was performed by RP-HPLC/MS/MS with positive electrospray ionization using multiple reaction monitoring. The calibration curve was linear in the range of 0.500-300 and 0.250-150 ng/mL for cilazapril and cilazaprilat, respectively. The proposed method was fully validated and proved to be selective, accurate, precise, reproducible, and suitable for the determination of cilazapril and cilazaprilat in human plasma. Therefore, it was applied to a bioequivalence study after per os administration of 2.5 mg tablet formulations of cilazapril.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Equivalencia Terapéutica
/
Cromatografía Líquida de Alta Presión
/
Cilazapril
/
Espectrometría de Masas en Tándem
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J AOAC Int
Año:
2011
Tipo del documento:
Article
País de afiliación:
Grecia