Mechanism of cell death resulting from DNA interstrand cross-linking in mammalian cells.
Cell Death Dis
; 2: e187, 2011 Aug 04.
Article
en En
| MEDLINE
| ID: mdl-21814285
ABSTRACT
DNA interstrand cross-links (ICLs) are critical cytotoxic lesions produced by cancer chemotherapeutic agents such as the nitrogen mustards and platinum drugs; however, the exact mechanism of ICL-induced cell death is unclear. Here, we show a novel mechanism of p53-independent apoptotic cell death involving prolonged cell-cycle (G(2)) arrest, ICL repair involving HR, transient mitosis, incomplete cytokinesis, and gross chromosomal abnormalities resulting from ICLs in mammalian cells. This characteristic 'giant' cell death, observed by using time-lapse video microscopy, was reduced in ICL repair ERCC1- and XRCC3-deficient cells. Collectively, the results illustrate the coordination of ICL-induced cellular responses, including cell-cycle arrest, DNA damage repair, and cell death.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Apoptosis
/
Reactivos de Enlaces Cruzados
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cell Death Dis
Año:
2011
Tipo del documento:
Article
País de afiliación:
Reino Unido