A simple method for introducing marker-free deletions in the Bacillus subtilis genome.
Methods Mol Biol
; 765: 345-58, 2011.
Article
en En
| MEDLINE
| ID: mdl-21815102
ABSTRACT
A genetic tool for introducing marker-free deletions is essential for multiple manipulations of genomes. We have developed a simple and efficient method for creating marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacillus subtilis
/
Transformación Bacteriana
/
ADN Recombinante
/
Genoma Bacteriano
/
Eliminación de Gen
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2011
Tipo del documento:
Article
País de afiliación:
Japón