Assessment of DNA integrity (COMET assay) in sperm cells of boron-exposed workers.
Arch Toxicol
; 86(1): 27-35, 2012 Jan.
Article
en En
| MEDLINE
| ID: mdl-21833739
ABSTRACT
An extension of a male reproductive study conducted in a boric acid/borate production zone at Bandirma, Turkey, is presented. The relation between DNA-strand breaks (COMET assay, neutral and alkaline version) in sperm cells and previously described sperm quality parameters was investigated in boron-exposed males. A correlation between blood boron levels and mean DNA-strand breaks in sperm was weak, and DNA-strand breaks in sperm were statistically not different between control and exposed groups. Therefore, increasing boron exposures had no additional contribution in addition to already pre-existing DNA-strand breaks in the sperm cells. Weak but statistically significant correlations between DNA-strand breaks and motility/morphology parameters of sperm samples were observed in the neutral version of the COMET assay, while correlations between the same variables were statistically not significant in the alkaline version. A likely reason for these negative results, even in highly exposed humans, is that experimental exposures that had led to reproductive toxicity in animals were significantly higher than any boron exposures, which may be reached under realistic human conditions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Boro
/
Exposición Profesional
/
Roturas del ADN
Límite:
Adult
/
Humans
/
Male
/
Middle aged
País/Región como asunto:
Asia
Idioma:
En
Revista:
Arch Toxicol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Turquía