Sequence homologies between Mycoplasma and Chlamydia spp. lead to false-positive results in chlamydial cell cultures tested for mycoplasma contamination with a commercial PCR assay.
J Clin Microbiol
; 49(10): 3681-2, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21849688
ABSTRACT
Mycoplasma contamination is a frequent problem in chlamydial cell culture. After obtaining contradictory contamination results, we compared three commercial PCR kits for mycoplasma detection. One kit signaled contamination in mycoplasma-free Chlamydia pneumoniae cultures. Sequencing of cloned PCR products revealed primer homology with the chlamydial genome as the basis of this false-positive result.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
/
Infecciones por Chlamydia
/
Chlamydia
/
Reacción en Cadena de la Polimerasa
/
Técnicas Bacteriológicas
/
Reacciones Falso Positivas
/
Mycoplasma
Límite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Austria