Cerebral oxygen metabolism of rats using injectable (15)O-oxygen with a steady-state method.
J Cereb Blood Flow Metab
; 32(1): 33-40, 2012 Jan.
Article
en En
| MEDLINE
| ID: mdl-21863038
ABSTRACT
To develop a less-stressful and simple method for measurement of the cerebral metabolic rate of oxygen (CMRO(2)) in small animals, the steady-state method was applied to injectable (15)O(2)-PET ((15)O(2)-positron emission tomography) using hemoglobin-containing vesicles ((15)O(2)-HbV). Ten normal rats and 10 with middle cerebral arterial occlusion (MCAO) were studied using a small animal PET scanner. A series of (15)O-PET scans with C(15)O-labeled HbV, H(2)(15)O, and (15)O(2)-HbV were performed with 10 to 15 minutes intervals to measure cerebral blood volume (CBV), cerebral blood flow (CBF), and CMRO(2). Positron emission tomography scans were started with a tracer injection using a multiprogramming syringe pump, which provides a slowly increasing injection volume to achieve steady-state radioactivity for H(2)(15)O and (15)O(2)-HbV scans. The radioactivity concentration of (15)O rapidly achieved equilibrium in the blood and whole brain at about 2 minutes after H(2)(15)O and (15)O(2)-HbV administration, which was stable during the scans. The whole brain mean values of CBF, CBV, and CMRO(2) were 54.3±2.0 mL per 100 g per minute, 4.9±0.4 mL/100 g, and 2.8±0.2 µmoL per g per minute (6.2±0.4 mL per 100 g per minute) in the normal rats, respectively. In the MCAO model rats, all hemodynamic parameters of the infarction area on the occlusion side significantly decreased. The steady-state method with (15)O-labeled HbV is simple and useful to analyze hemodynamic changes in studies with model animals.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxígeno
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Volumen Sanguíneo
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Corteza Cerebral
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Circulación Cerebrovascular
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Infarto de la Arteria Cerebral Media
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Tomografía de Emisión de Positrones
Límite:
Animals
Idioma:
En
Revista:
J Cereb Blood Flow Metab
Año:
2012
Tipo del documento:
Article
País de afiliación:
Japón