Selective translation of leaderless mRNAs by specialized ribosomes generated by MazF in Escherichia coli.
Cell
; 147(1): 147-57, 2011 Sep 30.
Article
en En
| MEDLINE
| ID: mdl-21944167
ABSTRACT
Escherichia coli (E. coli) mazEF is a stress-induced toxin-antitoxin (TA) module. The toxin MazF is an endoribonuclease that cleaves single-stranded mRNAs at ACA sequences. Here, we show that MazF cleaves at ACA sites at or closely upstream of the AUG start codon of some specific mRNAs and thereby generates leaderless mRNAs. Moreover, we provide evidence that MazF also targets 16S rRNA within 30S ribosomal subunits at the decoding center, thereby removing 43 nucleotides from the 3' terminus. As this region comprises the anti-Shine-Dalgarno (aSD) sequence that is required for translation initiation on canonical mRNAs, a subpopulation of ribosomes is formed that selectively translates the described leaderless mRNAs both in vivo and in vitro. Thus, we have discovered a modified translation machinery that is generated in response to MazF induction and that probably serves for stress adaptation in Escherichia coli.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
/
ARN Bacteriano
/
ARN Mensajero
/
Regulación Bacteriana de la Expresión Génica
/
Proteínas de Escherichia coli
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Proteínas de Unión al ADN
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Endorribonucleasas
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Escherichia coli
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Subunidades Ribosómicas Pequeñas Bacterianas
Idioma:
En
Revista:
Cell
Año:
2011
Tipo del documento:
Article
País de afiliación:
Austria