[Therapeutic effects of bone marrow transplantation on ovarian injury in mice].
Nan Fang Yi Ke Da Xue Xue Bao
; 31(9): 1534-8, 2011 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-21945760
ABSTRACT
OBJECTIVE:
To evaluate the therapeutic effects of bone marrow transplantation (BMT) on ovarian injury induced by chemotherapy in mice.METHODS:
Forty-eight mice were randomized equally into normal control group (A), cyclophosphamide and BMT group (B), and cyclophosphamide group (C). The mice in groups B and C were treated with intraperitoneal injection of cyclophosphamide at the daily dose of 150 mg/kg for 3 consecutive days, and allogeneic bone marrow cell transplantation was performed in group B. The ovary coefficient and the amount of follicles were compared to evaluate the function of ovaries. For cell tracking, the bone marrow cells were labeled with Hoechst 33342 and detected through fluorescence microscope after transplantation.RESULTS:
On days 21 and 50 after cyclophosphamide treatment, the ovary coefficient and the amount of follicles were significantly lowered in groups B and C (P<0.05), but the reduction was obviously ameliorated in group B (P<0.05). Cell tracking showed the presence of the donor bone marrow cells in the ovaries of the recipients mice after BMT.CONCLUSION:
BMT can improve the ovarian function impaired by chemotherapy in mice.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ovario
/
Trasplante de Médula Ósea
/
Ciclofosfamida
Límite:
Animals
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Año:
2011
Tipo del documento:
Article
País de afiliación:
China