Live-cell 3D super-resolution imaging in thick biological samples.

We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 µm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.