Expression and association of TRPC1 with TRPC3 during skeletal myogenesis.
Muscle Nerve
; 44(3): 358-65, 2011 Sep.
Article
en En
| MEDLINE
| ID: mdl-21996795
ABSTRACT
INTRODUCTION:
TRPC1 and TRPC3 proteins are widely expressed in skeletal muscles in forming calcium-permeable channels. Herein we characterize the expression pattern of TRPC transcripts during skeletal myogenesis in C2C12 myoblasts.METHODS:
We used polymerase chain reaction and Western blotting to detect expression levels, immunohistochemistry for subcellular localization, and co-immunoprecipitation techniques to assess interaction.RESULTS:
TRPC1 localizes to the cytoplasm and is enriched in the perinuclear region in undifferentiated myoblasts. Expression of TRPC1 increases significantly during myogenesis and resides mainly in differentiated myocytes and myotubes. TRPC3 is absent in undifferentiated myoblasts, is dramatically upregulated in differentiated culture, and is preferentially expressed in myotubes. Physical interaction of TRPC1-TRPC3 was observed, suggesting the possible existence of heteromers.CONCLUSIONS:
Expression of TRPC1 and TRPC3 is tightly regulated during myogensis. Evidence of TRPC1-TRPC3 interaction was first demonstrated in a muscle cell line. The functional consequences of this interaction remain to be established.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Desarrollo de Músculos
/
Mioblastos Esqueléticos
/
Canales Catiónicos TRPC
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Muscle Nerve
Año:
2011
Tipo del documento:
Article
País de afiliación:
Hong Kong