[Establishment of a novel cell model targeted on FGF-21 receptor for screening anti-diabetic drug candidates].
Yao Xue Xue Bao
; 46(8): 904-9, 2011 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-22007513
ABSTRACT
The aim of this project is to establish a fibroblast growth factor-21 (FGF-21) signaling pathway targeted cell model, for screening a class of FGF-21 receptor agonists as anti-diabetic candidates. FGF-21 requires beta klotho transmembrane proteins as co-receptor for the activation of tyrosine kinase FGF receptor (FGFR) signaling, thereby activating a series of intracellular signaling pathways and regulating gene transcription for glucose metabolism. Firstly a recombinant plasmid expressing co-receptor beta klotho and EGFP reporter genes was constructed. After introducing the recombinant plasmid into package cells, the cell culture supernatant was used to infect 3T3-L1 cells, which were then screened for stably expressing beta klotho gene. Administration of FGF-21 increased the expression of GLUT1 and stimulated GLUT1-mediated glucose uptake. This novel cell model can be conveniently used in high-throughput drug screening of FGF-21 or FGF-21 analogues.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores de Factores de Crecimiento de Fibroblastos
/
Factores de Crecimiento de Fibroblastos
/
Hipoglucemiantes
/
Proteínas de la Membrana
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Año:
2011
Tipo del documento:
Article
País de afiliación:
China