[Quantitative determination of contents of three components in Brucea javanica by HPLC].
Zhongguo Zhong Yao Za Zhi
; 36(14): 1979-81, 2011 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-22016972
ABSTRACT
OBJECTIVE:
To develop an HPLC method for quantitative determination of three quassinoids in Brucea javanic.METHOD:
The determination was carried out on a phenomenex C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution program of methol-water at a flow rate of 1.0 mL x min(-1), and the detection wavelength was 270 nm.RESULT:
Linearites of bruceine D, brusatol and bruceine H were good (r = 0.9996, 0.9996, and 0.9998) in ranges of 2.52-12.60, 2.19-10.95, and 2.91-14.55 microg, respectively. The average recoveries of bruceine D, brusatol and bruceine H were 100.01%, 100.95% and 100.43% respectively, and RSD of the above three compounds were 0.31% (n = 6), 1.7% ( n = 6) and 1.7% (n = 6), respectively.CONCLUSION:
The determination results of three batch samples showed that the method was simple, accurate and could be used in the quantitative determination of three components in the B. javanica.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Orgánicos
/
Cromatografía Líquida de Alta Presión
/
Brucea
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2011
Tipo del documento:
Article
País de afiliación:
China