Simultaneous determination of flucloxacillin and ampicillin in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and subsequent application to a clinical study in healthy Chinese volunteers.
J Pharm Biomed Anal
; 59: 157-61, 2012 Feb 05.
Article
en En
| MEDLINE
| ID: mdl-22040729
ABSTRACT
A novel method has been developed for the simultaneous determination of flucloxacillin and ampicillin in human plasma by ultra performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). The plasma was treated by single step of protein precipitation (PPT) with acetonitrile. The chromatographic separation was performed with a mobile phase consisting of 10mM ammonium formate and acetonitrile (6832, v/v). The analyses were carried out by multiple reaction monitoring (MRM) using the precursor-to-product combinations of m/z 454.1â160.3 (flucloxacillin), m/z 350.1â106.4 (ampicillin) and m/z 436.1â277.3 (IS). Validation results indicated that the lower limit of quantification (LLOQ) were both 0.2 µg/mL and both assay exhibited a linear range of 0.2-500 µg/mL. The intra-batch precision (R.S.D.) was less than 10.6% and inter-batch R.S.D. was less than 11.2%, while accuracy was with ±8% and ±9.9%, determined from QC samples for flucloxacillin and ampicillin. A rapid, sensitive and specific method for simultaneous quantifying flucloxacillin and ampicillin in human plasma have been devised and successfully applied to a clinic pharmacokinetic study.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masas en Tándem
/
Floxacilina
/
Ampicilina
/
Antibacterianos
Tipo de estudio:
Prognostic_studies
Límite:
Adult
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2012
Tipo del documento:
Article
País de afiliación:
China