Enhanced production of ß-carotene by recombinant industrial wine yeast using grape juice as substrate.
Curr Microbiol
; 64(2): 152-8, 2012 Feb.
Article
en En
| MEDLINE
| ID: mdl-22080204
ABSTRACT
In this study, both recombinant Saccharomyces cerevisiae T73-63 and FY-09 derived from the industrial wine yeast T73-4 and laboratory yeast FY1679-01B, respectively, were constructed and compared for their ß-carotene production in real grape juice. The results showed that highest ß-carotene content (5.89 mg/g) was found in strain T73-63, which was 2.1 fold higher than that of strain FY-09. Although the cell growth was inhibited by the metabolic burden induced by the production of heterogeneous ß-carotene, the pigment yield in T73-63 was still 1.7 fold higher than that of FY-09. Furthermore, high contents of ergosterol and fatty acid were also observed in T73-63. These results suggest that industrial wine yeast has highly active metabolic flux in mevalonate pathway, which leads to more carbon flux into carotenoid branch compared to that of laboratory yeast. The results of this study collectively suggest that in the application of recombinant strains to produce carotenoid using agro-industrial by-products as substrate, the suitable host strains should have active mevalonate pathway. For this purpose, the industrial wine yeast is a suitable candidate.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Vino
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Bebidas
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Beta Caroteno
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Vitis
Idioma:
En
Revista:
Curr Microbiol
Año:
2012
Tipo del documento:
Article