Gold nanoparticle-assembled capsules and their application as hydrogen peroxide biosensor based on hemoglobin.
Bioelectrochemistry
; 84: 32-7, 2012 Apr.
Article
en En
| MEDLINE
| ID: mdl-22112719
ABSTRACT
Gold nanoparticle-assembled capsules (GNACs) with controllable size and tunable morphology were fabricated through a simple two-step mixing procedure. Cationic polyelectrolyte was first induced to self-assemble into spherical aggregates in the presence of multivalent anions. Then, the aggregates served as an effective template for the self-assembly of gold nanoparticles to form size-controllable capsules. By adjusting the quantity of gold nanoparticles, capsules with various morphologies could be obtained. Because of their unique nanoporous features, the capsules with intact shells were further used to load hemoglobin (Hb) for the fabrication of a novel H(2)O(2) biosensor. The results of UV-vis spectroscopy and cyclic voltammetry indicated that the capsules provided a suitable matrix for the immobilization of Hb. Additionally, the resulting biosensor showed a high affinity and good catalytic activity to H(2)O(2). With the advantages of the large surface area, good conductivity and biocompatibility, the GNACs can offer a promising platform for the development of biosensors. Moreover, on the basis of the capsule structure, this material may also be expected to apply in some fields such as drug delivery, medical diagnostics and bio-encapsulation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hemoglobinas
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Técnicas Biosensibles
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Nanopartículas del Metal
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Oro
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Peróxido de Hidrógeno
Idioma:
En
Revista:
Bioelectrochemistry
Asunto de la revista:
BIOQUIMICA
Año:
2012
Tipo del documento:
Article