Isolation and characterization of microsatellite loci in a clonal herb, Cardamine leucantha (Brassicaceae).

ABSTRACT

PREMISE OF THE STUDY Microsatellite markers were developed to analyze the clonal diversity and population genetic structure of a clonal plant, Cardamine leucantha. METHODS AND RESULTS: Microsatellite loci were isolated by using compound simple sequence repeat (SSR) primers, resulting in 25 pairs including 14 useful markers. Samples from 33, 20, and 17 ramets in three populations were identified as belonging to 23, 18, and 17 genotypic individuals, respectively. CONCLUSIONS: These SSR markers will be used for identifying genets and evaluating genetic diversity of the clonal plant C. leucantha.