Effect of diallyl trisulfide derivatives on the induction of apoptosis in human prostate cancer PC-3 cells.
Mol Cell Biochem
; 363(1-2): 75-84, 2012 Apr.
Article
en En
| MEDLINE
| ID: mdl-22143535
ABSTRACT
The effects of five derivatives of diallyl trisulfide (DATS) were investigated on apoptosis in prostate cancer PC-3 cells, including dibutenyl trisulfide (DBTS), bis(2-methylallyl) trisulfide (2-M-DATS), dipentenyl trisulfide (DPTS), bis(3-methylbut-2-enyl) trisulfide (3-M-DBTS), and dihexenyl trisulfide (DHTS). Our present study demonstrated that DATS derivatives can suppress proliferation of PC-3 cells in a dose- and time-dependent manner, and that a change in the DATS structure could have an impact on its biological activity in the following order 2-M-DATS > DBTS ≈ DPTS ≈ DATS > 3-M-DBTS ≈ DHTS. Typical apoptotic nuclei were shown by Hoechst 33342 staining with 80 µM concentrations of DATS derivatives for 24 h. And flow cytometric analysis and DNA fragmentation assay also demonstrated that DATS derivatives induced apoptosis in PC-3 cells. Meanwhile, experimental results showed that DBTS, 2-M-DATS, and DPTS cause G2-M phase cell cycle arrest. Furthermore, a series of apoptosis-associated features were observed, which include a notable decrease in the expression of procaspases-3, up-regulation of pro-apoptotic proteins Bax expression, and down-regulation of anti-apoptotic proteins Bcl-2 expression in PC-3 cells. All of the evidences above indicate that DATS derivatives suppressed proliferation of PC-3 cells which was associated with the induction of apoptosis regulated by Bax/Bcl-2.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
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Sulfuros
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Apoptosis
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Compuestos Alílicos
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Antineoplásicos
Límite:
Humans
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Male
Idioma:
En
Revista:
Mol Cell Biochem
Año:
2012
Tipo del documento:
Article
País de afiliación:
China