Detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).
J Virol Methods
; 179(2): 330-4, 2012 Feb.
Article
en En
| MEDLINE
| ID: mdl-22155579
ABSTRACT
Reverse transcription loop-mediated isothermal amplification (RT-LAMP), which is a visual assay for nucleic acids, is performed in a single step using one tube at 65 °C for 1.5 h. In this study, RT-LAMP was established as a method for the detection of enterovirus 71 (EV71). The detection limit of the assay was approximately 10 copies, and no cross-reactivity was noted with Coxsackievirus A16, echovirus, human rotavirus (HRV) or norovirus. This assay, which offers greater sensitivity at a lower cost compared with the conventional reverse transcription polymerase chain reaction (RT-PCR), was validated using 252 clinical specimens that had been confirmed by laboratory diagnosis using RT-PCR. Both methods produced the same results with 52 positive samples. The RT-LAMP-based assay does not require specialised equipment, and therefore, it can be performed conveniently during an outbreak or under field conditions. In brief, the RT-LAMP-based assay provided a simple, rapid and efficient method for the detection of EV71 nucleic acid under field conditions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virología
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Técnicas de Amplificación de Ácido Nucleico
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Enterovirus Humano A
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Técnicas de Diagnóstico Molecular
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Infecciones por Enterovirus
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
/
Child
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Child, preschool
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Humans
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Infant
Idioma:
En
Revista:
J Virol Methods
Año:
2012
Tipo del documento:
Article
País de afiliación:
China