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Desmosomal cadherins in zebrafish epiboly and gastrulation.
Goonesinghe, Alexander; Luan, Xing-Ming; Hurlstone, Adam; Garrod, David.
Afiliación
  • Goonesinghe A; Faculty of Life Sciences, University of Manchester, (Michael Smith Building, Oxford Road), Manchester (M13 9PT), UK.
BMC Dev Biol ; 12: 1, 2012 Jan 11.
Article en En | MEDLINE | ID: mdl-22235774
BACKGROUND: The desmosomal cadherins (DCs), desmocollin (Dsc) and desmoglein (Dsg), are the adhesion molecules of desmosomes, intercellular adhesive junctions of epithelia and cardiac muscle. Both the DCs and desmosomes have demonstrably essential roles in mammalian development. In order to initiate their study in a more tractable developmental system we have characterised zebrafish DCs and examined their roles in early zebrafish development. RESULTS: We find that zebrafish possess one Dsc, the orthologue of mammalian Dsc1, which we designate zfDsc. Unlike mammalian Dscs, zfDsc exists only as the "a" form since it lacks the alternatively-spliced mini-exon that shortens the cytoplasmic domain to produce the "b" form. Zebrafish possess two Dsgs, designated zfDsgα and zfDsgß, orthologues of mammalian Dsg2. They show 43.8% amino acid identity and the α form has a 43 amino acid glycine-rich sequence of unknown function in its extracellular domain. Both zfDsc and zfDsgα were present as maternal and zygotic transcripts whereas zfDsgß was first expressed from 8 hours post-fertilisation (hpf). All three transcripts were present throughout subsequent stages of development. Morpholino knockdown of both zfDsc and zfDsgα expression produced similar defects in epiboly, axis elongation and somite formation, associated with abnormal desmosomes or reduced desmosome numbers. CONCLUSIONS: These results demonstrate an important role for DCs and desmosomes in the early morphogenesis of the zebrafish embryo, provide a basis for more detailed analysis of their role and raise interesting questions relating to the evolution and functional significance of DC isoforms.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Proteínas de Pez Cebra / Desmosomas / Desmocolinas / Desmogleínas / Gastrulación Límite: Animals Idioma: En Revista: BMC Dev Biol Asunto de la revista: EMBRIOLOGIA Año: 2012 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Proteínas de Pez Cebra / Desmosomas / Desmocolinas / Desmogleínas / Gastrulación Límite: Animals Idioma: En Revista: BMC Dev Biol Asunto de la revista: EMBRIOLOGIA Año: 2012 Tipo del documento: Article Pais de publicación: Reino Unido