The urea-soluble low molecular weight cuticle proteins from the different developmental stages of Dacus oleae.

Souliotis, V L; Patrinou-Georgoula, M; Zongza, V; Dimitriadis, G J

Souliotis, V L; Patrinou-Georgoula, M; Zongza, V; Dimitriadis, G J.

Afiliación

Souliotis VL; National Hellenic Research Foundation, University of Athens, Greece.

Cell Differ Dev ; 31(1): 23-9, 1990 Jul.

Article en En | MEDLINE | ID: mdl-2224572

ABSTRACT

ABSTRACT

The cuticle proteins of the insect Dacus oleae have been isolated by extraction with a solution of 7 M urea. The affinity properties of cuticle proteins, isolated from the third instar larvae (L3DCPs 1-7), to chitin have been studied. Purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. The developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis.

Asunto(s)

Dípteros/crecimiento & desarrollo; Proteínas de Insectos; Proteínas/aislamiento & purificación; Animales; Anticuerpos; Quitina; Cromatografía de Afinidad; Dípteros/análisis; Electroforesis en Gel Bidimensional; Peso Molecular; Solubilidad; Urea

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Proteínas de Insectos / Dípteros Límite: Animals Idioma: En Revista: Cell Differ Dev Asunto de la revista: EMBRIOLOGIA Año: 1990 Tipo del documento: Article País de afiliación: Grecia

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