Adipose tissue remodeling in children: the link between collagen deposition and age-related adipocyte growth.
J Clin Endocrinol Metab
; 97(4): 1320-7, 2012 Apr.
Article
en En
| MEDLINE
| ID: mdl-22259057
ABSTRACT
CONTEXT Extracellular matrix (ECM) remodeling is essential for adipose tissue growth and expansion in high fat-fed mice, and there is evidence of fibrosis in adipose tissue in human obesity. OBJECTIVE:
The aim of the study was to explore the role of ECM remodeling in adipose tissue in healthy, growing children. RESEARCH DESIGN, SETTING, ANDPARTICIPANTS:
Abdominal sc adipose biopsies were obtained from 65 otherwise healthy children [57 boys; age, 5.3 ± 3.8 yr (mean ± sd)] having elective surgery (cross-sectional study). Twenty percent of the participants were classified as overweight/obese based on body mass index (BMI) z score. MAIN OUTCOMEMEASURES:
We examined collagen (total and pericellular), HAM56+ macrophages, CD206+ M2 phenotype macrophages, and CD3+ T cells measured by immunohistochemistry and ECM gene expression markers.RESULTS:
Overweight children had significantly less total collagen compared to normal weight children (median, 3.4 vs. 9.1%; P = 0.001). However, collagen areas were not positive for COL6 and showed little evidence of collagen surrounding adipocytes. Fat cell size was negatively correlated with the percentage of total (r = -0.398; P = 0.003) and pericellular collagen (r = -0.462; P < 0.001) but positively correlated with HAM56+ macrophages (r = 0.541; P < 0.001). The percentage of total collagen was inversely associated with BMI z score (r = -0.345; P = 0.01) and age (r = -0.348; P = 0.005), with older (>11 yr old) children in the top BMI z tertile having less collagen (3.8%) than younger (2-5 yr old) children in the bottom BMI z tertile (12.6%). Adipose tissue in overweight children showed little evidence of crown-like structures or T cells.CONCLUSION:
In healthy, growing children, increased collagen in adipose tissue is associated with decreased fat cell size and BMI z score and increased M2+ phenotype macrophages, suggesting dynamic interaction between ECM remodeling and immune cells even at an early age.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Desarrollo Infantil
/
Colágeno
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Grasa Subcutánea Abdominal
/
Sobrepeso
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Adipogénesis
/
Matriz Extracelular
Tipo de estudio:
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Límite:
Child
/
Child, preschool
/
Female
/
Humans
/
Male
País/Región como asunto:
Oceania
Idioma:
En
Revista:
J Clin Endocrinol Metab
Año:
2012
Tipo del documento:
Article
País de afiliación:
Australia