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Two-step mechanism for modifier of transcription 1 (Mot1) enzyme-catalyzed displacement of TATA-binding protein (TBP) from DNA.
Moyle-Heyrman, Georgette; Viswanathan, Ramya; Widom, Jonathan; Auble, David T.
Afiliación
  • Moyle-Heyrman G; Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, USA.
J Biol Chem ; 287(12): 9002-12, 2012 Mar 16.
Article en En | MEDLINE | ID: mdl-22298788
ABSTRACT
The TATA box binding protein (TBP) is a central component of the transcription preinitiation complex, and its occupancy at a promoter is correlated with transcription levels. The TBP-promoter DNA complex contains sharply bent DNA and its interaction lifetime is limited by the ATP-dependent TBP displacement activity of the Snf2/Swi2 ATPase Mot1. Several mechanisms for Mot1 action have been proposed, but how it catalyzes TBP removal from DNA is unknown. To better understand the Mot1 mechanism, native gel electrophoresis and FRET were used to determine how Mot1 affects the trajectory of DNA in the TBP-DNA complex. Strikingly, in the absence of ATP, Mot1 acts to unbend DNA, whereas TBP remains closely associated with the DNA in a stable Mot1-TBP-DNA ternary complex. Interestingly, and in contrast to full-length Mot1, the isolated Mot1 ATPase domain binds DNA, and its affinity for DNA is nucleotide-dependent, suggesting parallels between the Mot1 mechanism and DNA translocation-based mechanisms of chromatin remodeling enzymes. Based on these findings, a model is presented for Mot1 that links a DNA conformational change with ATP-induced DNA translocation.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / ADN de Hongos / Adenosina Trifosfatasas / Proteínas de Saccharomyces cerevisiae / Factores Asociados con la Proteína de Unión a TATA / Proteína de Unión a TATA-Box Idioma: En Revista: J Biol Chem Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / ADN de Hongos / Adenosina Trifosfatasas / Proteínas de Saccharomyces cerevisiae / Factores Asociados con la Proteína de Unión a TATA / Proteína de Unión a TATA-Box Idioma: En Revista: J Biol Chem Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos