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Identification of an immunomodulating metalloprotease of Pseudomonas aeruginosa (IMPa).
Bardoel, Bart W; Hartsink, Dennis; Vughs, Mignon M; de Haas, Carla J C; van Strijp, Jos A G; van Kessel, Kok P M.
Afiliación
  • Bardoel BW; Department of Medical Microbiology, University Medical Center Utrecht, 3584CX Utrecht, The Netherlands. b.w.bardoel@umcutrecht.nl
Cell Microbiol ; 14(6): 902-13, 2012 Jun.
Article en En | MEDLINE | ID: mdl-22309196
Phagocytosis by neutrophils is the essential step in fighting Pseudomonas infections. The first step in neutrophil recruitment to the site infection is the interaction of P-selectin (on endothelial cells) with P-selectin glycoprotein ligand-1 (PSGL-1) on neutrophils. Pseudomonas aeruginosa secretes various proteases that degrade proteins that are essential for host defence, such as elastase and alkaline protease. Here we identify PA0572 of P. aeruginosa as an inhibitor of PSGL-1 and named this secreted hypothetical protease immunomodulating metalloprotease of P. aeruginosa or IMPa. Proteolytic activity was confirmed by cleavage of recombinant and cell-surface expressed PSGL-1. Functional inhibition was demonstrated by impaired PSGL-1-mediated rolling of IMPa-treated neutrophils under flow conditions. Next to PSGL-1, IMPa targets CD43 and CD44 that are also involved in leucocyte homing. These data indicate that IMPa prevents neutrophil extravasation and thereby protects P. aeruginosa from neutrophil attack.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Proteínas Bacterianas / Metaloproteasas / Inmunomodulación Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Cell Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2012 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: India

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Proteínas Bacterianas / Metaloproteasas / Inmunomodulación Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Cell Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2012 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: India