Metabolite identification and quantitation in LC-MS/MS-based metabolomics.
Trends Analyt Chem
; 32: 1-14, 2012 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-22345829
Metabolomics aims at detection and quantitation of all metabolites in biological samples. The presence of metabolites with a wide variety of physicochemical properties and different levels of abundance challenges existing analytical platforms used for identification and quantitation of metabolites. Significant efforts have been made to improve analytical and computational methods for metabolomics studies.This review focuses on the use of liquid chromatography with tandem mass spectrometry (LC-MS/MS) for quantitative and qualitative metabolomics studies. It illustrates recent developments in computational methods for metabolite identification, including ion annotation, spectral interpretation and spectral matching. We also review selected reaction monitoring and high-resolution MS for metabolite quantitation. We discuss current challenges in metabolite identification and quantitation as well as potential solutions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Idioma:
En
Revista:
Trends Analyt Chem
Año:
2012
Tipo del documento:
Article
Pais de publicación:
Países Bajos