Microsatellite loci for Gossypium davidsonii (Malvaceae) and other D-genome, Sonoran Desert endemic cotton species.
Am J Bot
; 99(3): e91-3, 2012 Mar.
Article
en En
| MEDLINE
| ID: mdl-22358044
ABSTRACT
PREMISE OF THE STUDY Microsatellite primers previously developed for domesticated cotton (Gossypium hirsutum; tetraploid) were screened for their utility in investigating genetic structure and gene flow within G. davidsonii and five other wild, Mexican, D-genome cotton species (all diploid). METHODS AND RESULTS:
We screened 50 microsatellite primer pairs from the Cotton Marker Database, identifying 10 loci as polymorphic within G. davidsonii. In genotyping approximately 200 individuals from four populations, we found that the number of alleles per locus ranged from seven to 17, and mean observed and expected heterozygosity ranged from 0.145 to 0.492 and from 0.436 to 0.734, respectively. We genotyped six to 20 individuals from each of the remaining species, finding these 10 loci to cross-amplify in all cases and in most cases to be polymorphic.CONCLUSIONS:
These markers may be useful for further investigation of population genetics of G. davidsonii and other wild D-genome cotton species.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genoma de Planta
/
Repeticiones de Microsatélite
/
Gossypium
/
Clima Desértico
Tipo de estudio:
Prognostic_studies
País/Región como asunto:
Mexico
Idioma:
En
Revista:
Am J Bot
Año:
2012
Tipo del documento:
Article