Lmx1b can promote the differentiation of embryonic stem cells to dopaminergic neurons associated with Parkinson's disease.
Biotechnol Lett
; 34(7): 1167-74, 2012 Jul.
Article
en En
| MEDLINE
| ID: mdl-22426839
ABSTRACT
The introduction of genes associated with the fate of dopaminergic (DA) neurons may help to facilitate the differentiation of embryonic stem cells (ESC) to DA neurons. Here we used lentiviral vectors to drive lmx1b, a key transcription factor of dopaminergic neurons development, expression in ESC. Tyrosine hydroxylase-positive neurons were increased from 18% to nearly 50% when exogenous lmx1b was transduced. The enhanced Pitx3, Girk2, Nurr1 and DAT suggested that the increased differentiated neurons were mature A9 DA neurons. The results of [(3)H]dopamine reuptake assay indicated these differentiated neurons were all functional. Our study demonstrated that lmx1b was capable of promoting the differentiation of ESC to DA neurons.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedad de Parkinson
/
Factores de Transcripción
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Diferenciación Celular
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Células Madre Embrionarias
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Neuronas Dopaminérgicas
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Proteínas con Homeodominio LIM
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biotechnol Lett
Año:
2012
Tipo del documento:
Article