Remotely activated protein-producing nanoparticles.
Nano Lett
; 12(6): 2685-9, 2012 Jun 13.
Article
en En
| MEDLINE
| ID: mdl-22432731
ABSTRACT
The development of responsive nanomaterials, nanoscale systems that actively respond to stimuli, is one general goal of nanotechnology. Here we develop nanoparticles that can be controllably triggered to synthesize proteins. The nanoparticles consist of lipid vesicles filled with the cellular machinery responsible for transcription and translation, including amino acids, ribosomes, and DNA caged with a photolabile protecting group. These particles served as nanofactories capable of producing proteins including green fluorescent protein (GFP) and enzymatically active luciferase. In vitro and in vivo, protein synthesis was spatially and temporally controllable, and could be initiated by irradiating micrometer-scale regions on the time scale of milliseconds. The ability to control protein synthesis inside nanomaterials may enable new strategies to facilitate the study of orthogonal proteins in a confined environment and for remotely activated drug delivery.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Robótica
/
Ingeniería de Proteínas
/
Proteínas
/
Cristalización
/
Nanoestructuras
Idioma:
En
Revista:
Nano Lett
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos