Detection of multiple genotypes of calicivirus infection in asymptomatic swine in Taiwan.
Zoonoses Public Health
; 59(6): 434-44, 2012 Sep.
Article
en En
| MEDLINE
| ID: mdl-22489630
ABSTRACT
Noroviruses (NoVs) and sapoviruses (SaVs) of the family Caliciviridae are emerging enteric pathogens in humans and animals. Recent detection of genogroup II norovirus (GII NoV) RNA from swine raises public health concerns about zoonotic transmission of porcine NoVs to humans. However, few papers reported genotype distributions and epidemiological features in swine farms and their genetic relationship to human strains, which was the objective of our study. This study investigated the epidemiological features and genotypes of caliciviruses in swine farms using 533 pig faecal samples from six farms in central and southern Taiwan, tested for viral RNA using RT-PCR targeting the conserved polymerase gene. NoVs and SaVs were detected with a positive rate of 7.1% and 0.6%, respectively. To confirm the positive rate of NoVs, 255 pig faecal samples from two farms in central Taiwan were tested with primer pairs targeting the partial capsid gene of GII, and 32.3% of the positive rate was found. Furthermore, the results from the capsid region suggested a higher positive rate of 41.7% in winter than 26.4% in summer with statistical significance (P < 0.05). Sequence analysis showed 29 strains belonging to GII.4 (human) and nine strains belonging to GII.11 (swine) identified based on the partial polymerase gene. Additional genotypes clustered with GII.2 (human) and GII.18 (swine) were also characterized based on the partial capsid gene. SaVs detected in porcine faecal samples belonged to genogroup III (GIII), which clustered with the PEC-Cowden strain. Our study demonstrated the presence of multiple genotypes of both human and porcine NoVs infecting swine of various ages asymptomatically. Although the zoonotic potential of detected human NoVs in swine was not conclusive owing to the lack of local human faecal samples, our study revealed the importance of monitoring emerging strains in swine to mitigate the potential impact of recombinant NoVs infecting the human population.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Porcinos
/
ARN Polimerasa Dependiente del ARN
/
Infecciones por Caliciviridae
/
Norovirus
/
Sapovirus
/
Proteínas de la Cápside
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Zoonoses Public Health
Asunto de la revista:
MEDICINA VETERINARIA
/
SAUDE PUBLICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Taiwán