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Production of prostaglandin E2 in response to infection with modified vaccinia Ankara virus.
Pollara, Justin J; Spesock, April H; Pickup, David J; Laster, Scott M; Petty, Ian T D.
Afiliación
  • Pollara JJ; Department of Microbiology, North Carolina State University, Raleigh, NC 27695, USA.
Virology ; 428(2): 146-55, 2012 Jul 05.
Article en En | MEDLINE | ID: mdl-22534090
ABSTRACT
Prostaglandin E2 (PGE2) is an arachidonic acid (AA)-derived signaling molecule that can influence host immune responses to infection or vaccination. In this study, we investigated PGE2 production in vitro by cells infected with the poxvirus vaccine strain, modified vaccinia Ankara virus (MVA). Human THP-1 cells, murine bone marrow-derived dendritic cells, and murine C3HA fibroblasts all accumulated PGE2 to high levels in culture supernatants upon infection with MVA. We also demonstrated that MVA induced the release of AA from infected cells, and this was, most unusually, independent of host cytosolic phospholipase A2 activity. The accumulation of AA and PGE2 was dependent on viral gene expression, but independent of canonical NF-κB signaling via p65/RelA. The production of PGE2 required host cyclooxygenase-2 (COX-2) activity, and COX-2 protein accumulated during MVA infection. The results of this study provide insight into a novel aspect of MVA biology that may affect the efficacy of MVA-based vaccines.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vaccinia / Virus Vaccinia / Dinoprostona Límite: Animals / Humans Idioma: En Revista: Virology Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vaccinia / Virus Vaccinia / Dinoprostona Límite: Animals / Humans Idioma: En Revista: Virology Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos