One-step amino acid selective isotope labeling of proteins in prototrophic Escherichia coli strains.
Anal Biochem
; 426(2): 126-8, 2012 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-22538396
ABSTRACT
Amino acid selective isotope labeling is a useful approach to simplification of nuclear magnetic resonance (NMR) spectra of large proteins. Cell-free protein synthesis offers essentially unlimited flexibility of labeling patterns but is labor-intensive and expensive. In vivo labeling is simple in principle but generally requires auxotrophic strains, inhibitors of amino acid synthesis, or complex media formulations. We describe a simple procedure for amino acid selective labeling of proteins expressed in prototrophic Escherichia coli strains. Excellent labeling selectivity was achieved for histidine, lysine, methionine, and alanine. Simplicity and robustness of this protocol make it a useful tool for protein NMR.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
/
Escherichia coli
/
Aminoácidos
/
Marcaje Isotópico
Idioma:
En
Revista:
Anal Biochem
Año:
2012
Tipo del documento:
Article