Comparison of the properties of purified mitochondrial and cytosolic rat kidney transamidinase.
Int J Biochem
; 22(11): 1243-50, 1990.
Article
en En
| MEDLINE
| ID: mdl-2257950
ABSTRACT
1. Mitochondrial rat kidney transamidinase was solubilized by two extractions with the surfactant Zwittergent 3-14. 2. Mitochondrial and cytosolic forms of rat kidney transamidinase were purified by chromatography on DEAE-Trisacryl M, phenyl-Sepharose Cl-4B and hydroxylapatite columns. 3. The specific activity of purified mitochondrial enzyme was significantly higher than purified cytosolic enzyme. 4. The subunit molecular mass, the electrophoretic mobility under nondenaturing conditions, and the activation energy were similar for purified mitochondrial and cytosolic transamidinase.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Amidinotransferasas
/
Riñón
Límite:
Animals
Idioma:
En
Revista:
Int J Biochem
Año:
1990
Tipo del documento:
Article