Dissecting and culturing and imaging the mouse urogenital system.
Methods Mol Biol
; 886: 3-11, 2012.
Article
en En
| MEDLINE
| ID: mdl-22639246
ABSTRACT
Current knowledge of the morphological and molecular events driving branching morphogenesis of the ureteric bud (UB) during development of the metanephric kidney has been greatly facilitated by the ability to explant this organ to culture. The UB can be further isolated from the mesenchyme and grown within a three-dimensional, collagen-based matrix when supplemented with the appropriate growth factors. The protocol presented here outlines the dissection and culture techniques necessary to dissect and culture the whole kidney and the isolated UB.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Órganos
/
Disección
/
Riñón
/
Ratones
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos