Isotopic profiling of ¹³C-labeled biological samples by two-dimensional heteronuclear J-resolved nuclear magnetic resonance spectroscopy.
Anal Biochem
; 427(2): 158-63, 2012 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-22658961
ABSTRACT
The use of two-dimensional heteronuclear J-resolved (2D H-JRES) nuclear magnetic resonance (NMR) spectroscopy for fast and reliable measurement of isotopic patterns from ¹³C-enriched compounds resulting from carbon labeling experiments was evaluated. Its use with biological samples of increasing complexity showed that 2D H-JRES spectroscopy is suitable for high-throughput isotopic profiling of any kind of labeled samples. Moreover, the method enabled accurate quantification of ¹³C enrichments and, thus, can be used for metabolic flux analysis. The excellent trade-off between reduced experimental time and the number of measurable isotopic data makes 2D H-JRES NMR a promising approach for high-throughput flux analysis of samples of intermediate complexity.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trypanosoma brucei brucei
/
Mezclas Complejas
/
Escherichia coli
/
Ensayos Analíticos de Alto Rendimiento
/
Aminoácidos
Idioma:
En
Revista:
Anal Biochem
Año:
2012
Tipo del documento:
Article
País de afiliación:
Francia