Chemical crosslinking and mass spectrometric identification of interaction sites within soluble aggregate of protein therapeutics.
J Pharm Biomed Anal
; 73: 99-102, 2013 Jan 25.
Article
en En
| MEDLINE
| ID: mdl-22677652
ABSTRACT
Protein aggregation presents a major challenge to bioengineering. In the present study, chemical crosslinking and mass spectrometry are employed to probe the interaction amongst soluble oligomer formed by Fc molecule of monoclonal antibody. Aggregation was induced by thermal stress at 42°C for 6h under physiological pH. Contacting residues on adjacent molecules were captured with bifunctional crosslinker BS3, followed by mass spectrometric identification of the crosslinked sites. The approach provides site-specific information regarding the binding interface that would have broad application in the field.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Mapeo Peptídico
/
Fragmentos Fc de Inmunoglobulinas
/
Reactivos de Enlaces Cruzados
/
Multimerización de Proteína
/
Factores Inmunológicos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos