[Effect comparison of Wright-Giemsa stain under different conditions and its apply in cell micronucleus test].

OBJECTIVE: To apply the Wright-Giemsa stain in micronucleus test and to explore the stain outcomes of Wright-Giemsa dye of various proportions and staining times. METHODS: Use Wright-Giemsa dye, Wright dye (staining time 3 min) and Giemsa dye (staining time 5 min) to stain HepG2 and then observe the staining effect. The Wright-Giemsa dye was applied under 5 different proportions (3:1-1:3) and different staining times (1, 3, 5, 10, 15 min). RESULTS: After stained for 3-5 min with the proportion ratio of 3:1 of Wright-Giemsa dye, the HepG2 cells showed much better staining outcomes compared with the single stain of either Wright or Giemsa. CONCLUSIONS: Wright-Giemsa stain can be used in cell micronucleus test to obtain good staining outcomes.