[Effect of shengbanfang on CYP3A1 activities of Sprague-Dawley rat].
Zhong Yao Cai
; 35(2): 254-9, 2012 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-22822673
ABSTRACT
OBJECTIVE:
To study the influences of Shengbanfang on CYP3A1 activities of SD rat and provide suggestions for drug combinations.METHODS:
25 male SD rats were devided into 5 groups randomly,and treated with saline( NS, ig, 10 mg/(kg/d) ,qd,14 d), dexamethasone (DEX, ig, 100 mg/(kg x d), qd, 3 d), high dose of Shengbanfang (HD, ig, 8.645 g/kg, bid, 14 d), middle dose of Shengbanfang (MD, ig, 4.322 g/kg, bid, 14 d) and low dose of Shengbanfang (LD, ig, 2.161 g/kg, bid, 14 d), respectively. The HPLC method was established and validated to determine the productive velocity of 6beta-hydroxytestosterone and measure the activity of CYP3Al.RESULTS:
Under the optimized incubation conditions, the productive rates of 6beta-hydroxytestosterone of HD, MD, LD, NS and DEX, groups were (55.82 +/- 5.97), (65.10 +/- 6.83), (60.89 +/- 6.53), (62.17 +/- 6.55), (126.73 +/- 15.40) micromol/(L x mg pro x min). There were significant differences between Shengbanfang groups compared with dexamethasone group, but there was no significant difference between Shengbanfang groups and the control group (NS).CONCLUSION:
Shengbanfang has no induce effect on the enzymic activity of CYP3Al in SD rats.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Testosterona
/
Dexametasona
/
Microsomas Hepáticos
/
Medicamentos Herbarios Chinos
/
Citocromo P-450 CYP3A
Límite:
Animals
Idioma:
Zh
Revista:
Zhong Yao Cai
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2012
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
CHINA
/
CN
/
REPUBLIC OF CHINA