Interlaboratory variation in a diaminopimelic acid assay: influence on estimated duodenal bacterial nitrogen flow.
J Dairy Sci
; 73(10): 2929-33, 1990 Oct.
Article
en En
| MEDLINE
| ID: mdl-2283421
ABSTRACT
Samples of ruminal bacteria and duodenal digesta were collected from two dairy cows fed a 65% forage diet. Samples were sent blind to four laboratories for diaminopimelic acid analysis. Analyzed values differed among laboratories within sample type, and concentrations ranked as follows laboratory D greater than laboratory A greater than laboratory B greater than laboratory C. Consideration of differences in actual procedures used among laboratories resulted in several hypotheses to explain some of the interlaboratory variation. Using diaminopimelic acid values from each laboratory to estimate duodenal bacterial nitrogen flow showed that laboratory D estimated a 17% higher flow than the average for laboratories A, B, and C, which were similar.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Bovinos
/
Ácido Diaminopimélico
/
Duodeno
/
Contenido Digestivo
Límite:
Animals
Idioma:
En
Revista:
J Dairy Sci
Año:
1990
Tipo del documento:
Article
País de afiliación:
Canadá