Biocytin-derived MRI contrast agent for longitudinal brain connectivity studies.
ACS Chem Neurosci
; 2(10): 578-87, 2011 Oct 19.
Article
en En
| MEDLINE
| ID: mdl-22860157
ABSTRACT
To investigate the connectivity of brain networks noninvasively and dynamically, we have developed a new strategy to functionalize neuronal tracers and designed a biocompatible probe that can be visualized in vivo using magnetic resonance imaging (MRI). Furthermore, the multimodal design used allows combined ex vivo studies with microscopic spatial resolution by conventional histochemical techniques. We present data on the functionalization of biocytin, a well-known neuronal tract tracer, and demonstrate the validity of the approach by showing brain networks of cortical connectivity in live rats under MRI, together with the corresponding microscopic details, such as fibers and neuronal morphology under light microscopy. We further demonstrate that the developed molecule is the first MRI-visible probe to preferentially trace retrograde connections. Our study offers a new platform for the development of multimodal molecular imaging tools of broad interest in neuroscience, that capture in vivo the dynamics of large scale neural networks together with their microscopic characteristics, thereby spanning several organizational levels.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biotina
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Encéfalo
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Imagen por Resonancia Magnética
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Medios de Contraste
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Complejos de Coordinación
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Lisina
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Vías Nerviosas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
ACS Chem Neurosci
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania