Label-free visualization of ultrastructural features of artificial synapses via cryo-EM.
ACS Chem Neurosci
; 2(12): 700-4, 2011 Dec 21.
Article
en En
| MEDLINE
| ID: mdl-22860164
ABSTRACT
The ultrastructural details of presynapses formed between artificial substrates of submicrometer silica beads and hippocampal neurons are visualized via cryo-electron microscopy (cryo-EM). The silica beads are derivatized by poly-d-lysine or lipid bilayers. Molecular features known to exist at presynapses are clearly present at these artificial synapses, as visualized by cryo-EM. Key synaptic features such as the membrane contact area at synaptic junctions, the presynaptic bouton containing presynaptic vesicles, as well as microtubular structures can be identified. This is the first report of the direct, label-free observation of ultrastructural details of artificial synapses.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sinapsis
/
Microscopía por Crioelectrón
Límite:
Animals
Idioma:
En
Revista:
ACS Chem Neurosci
Año:
2011
Tipo del documento:
Article
País de afiliación:
Canadá