Biofunctionalized magnetic nanoparticles for specifically detecting biomarkers of Alzheimer's disease in vitro.
ACS Chem Neurosci
; 2(9): 500-5, 2011 Sep 21.
Article
en En
| MEDLINE
| ID: mdl-22860173
ABSTRACT
Magnetic nanoparticles biofunctionalized with antibodies against ß-amyloid-40 (Aß-40) and Aß-42, which are promising biomarkers related to Alzheimer's disease (AD), were synthesized. We characterized the size distribution, saturated magnetizations, and stability of the magnetic nanoparticles conjugated with anti-Aß antibody. In combination with immunomagnetic reduction technology, it is demonstrated such biofunctionalized magnetic nanoparticles are able to label Aßs specifically. The ultralow-detection limits of assaying Aßs in vitro using the magnetic nanoparticles via immunomagnetic reduction are determined to a concentration of â¼10 ppt (10 pg/mL). Further, immunomagnetic reduction signals of Aß-40 and Aß-42 in human plasma from normal samples and AD patients were analyzed, and the results showed a significant difference between these two groups. These results show the feasibility of using magnetic nanoparticles with Aßs as reagents for assaying low-concentration Aßs through immunomagnetic reduction, and also provide a promising new method for early diagnosis of Alzheimer's disease from human blood plasma.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nanopartículas
/
Enfermedad de Alzheimer
/
Magnetismo
Tipo de estudio:
Screening_studies
Límite:
Humans
Idioma:
En
Revista:
ACS Chem Neurosci
Año:
2011
Tipo del documento:
Article
País de afiliación:
Taiwán