Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of colonies using the met30 strain.
J Biosci Bioeng
; 114(6): 619-21, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-22889581
Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Azufre
/
Color
/
Proteínas de Saccharomyces cerevisiae
/
Complejos de Ubiquitina-Proteína Ligasa
/
Proteínas F-Box
/
Genes Fúngicos
/
Mutación
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
J Biosci Bioeng
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Rusia
Pais de publicación:
Japón