A plausible anti-apoptotic role of up-regulated OCT4B1 in bladder tumors.
Urol J
; 9(3): 574-80, 2012.
Article
en En
| MEDLINE
| ID: mdl-22903480
ABSTRACT
PURPOSE:
To investigate and compare the expression of OCT4B1 between tumor and non-tumor bladder tissues. MATERIALS ANDMETHODS:
We investigated the expression of OCT4B1 in 30 tumor and non-tumor surgical specimens of the bladder, using the TaqMan real-time polymerase chain reaction approach and by carefully designing primers and probes specific for the amplification of the variant.RESULTS:
Most tumor and non-tumor samples of the bladder showed OCT4B1 expression, but its expression level was significantly higher in the tumors (P < .002). Moreover, the up-regulation of OCT4B1 was more significant in high-grade tumors compared to the low-grade ones (P < .05). We have also employed the RNA interference strategy to evaluate the functional role of OCT4B1 in a bladder cancer cell line, 5637. Suppression of OCT4B1 caused some changes in cell cycle distribution, and significantly elevated the rate of apoptosis in the cells.CONCLUSION:
Our findings suggest that OCT4B1 plays a potential role in tumor initiation and/or progression of the bladder cancer. Additionally, OCT4B1 can be regarded as a new tumor marker for detection, classification, and treatment of the bladder cancer. However, more experimental studies are needed to replicate our findings.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Vejiga Urinaria
/
Biomarcadores de Tumor
/
Factor 3 de Transcripción de Unión a Octámeros
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Urol J
Asunto de la revista:
UROLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Irán