A multiplex assay to detect variations in the CYP2C9, VKORC1, CYP4F2 and APOE genes involved in acenocoumarol metabolism.
Clin Biochem
; 46(1-2): 167-9, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-22917719
ABSTRACT
OBJECTIVES:
We have developed a genotyping system to determine the alleles of genes related to interindividual variability in acenocoumarol dosage requirements. This genotyping system is intended for routine clinical use and therefore it is essential that it be simple, fast and inexpensive. DESIGN ANDMETHODS:
We developed a PCR multiplex SNaPshot reaction that targets 6 SNPs (single nucleotide polymorphisms) in CYP2C9, CYP4F2, VKORC1 and APOE genes, which are associated with acenocoumarol dose requirements.RESULTS:
We tested the multiplex in 152 samples and found it to be 100% concordant with the results of other methods.CONCLUSIONS:
We successfully produced a reliable multiplex system for simultaneously typing 6 SNPs. This system may be used as a model for accurate, simple and inexpensive genotyping of SNPs related to dose requirements. This information allows the prediction of drug efficiency in patients prior to treatment with acenocoumarol and the prevention of adverse drug reactions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Apolipoproteínas E
/
Hidrocarburo de Aril Hidroxilasas
/
Polimorfismo de Nucleótido Simple
/
Sistema Enzimático del Citocromo P-450
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Reacción en Cadena de la Polimerasa Multiplex
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Oxigenasas de Función Mixta
/
Acenocumarol
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Clin Biochem
Año:
2013
Tipo del documento:
Article
País de afiliación:
España