Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3.
FEBS Lett
; 586(19): 3522-8, 2012 Sep 21.
Article
en En
| MEDLINE
| ID: mdl-22922005
ABSTRACT
ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ubiquitinas
/
Proteínas Activadoras de GTPasa
/
Proteínas Modificadoras Pequeñas Relacionadas con Ubiquitina
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2012
Tipo del documento:
Article
País de afiliación:
Brasil