Downregulated gene expression of TGF-ßs in diabetic oral wound healing.
J Craniomaxillofac Surg
; 41(2): e42-8, 2013 Mar.
Article
en En
| MEDLINE
| ID: mdl-22964221
ABSTRACT
BACKGROUND:
Healing of tooth extraction sockets in poorly controlled diabetic patients is often delayed and accompanied by severe infection. The exact cellular and molecular mechanisms underlying the pathogenesis of this complication are still not fully understood.OBJECTIVES:
The purpose of this study was to investigate molecular changes associated with delayed oral wound healing in diabetes. MATERIALS ANDMETHODS:
Six to eight weeks old male type 2 diabetes and age matched control inbred mice were used and maxillary molar tooth extractions were performed. At 4 and 7 days after tooth extraction, the edentulous mucosa of the mice were harvested, and analyzed for histology and gene expression of key wound healing factors.RESULTS:
In the diabetic model, histological analysis showed that epithelial tissue migration for wound closure was delayed after tooth extraction compared to the control. Quantitative real-time PCR revealed that expression of the TGF-ß1, TGF-ß2, TGF-ß3, TGFßRII and TGFßRIII genes was significantly downregulated in the diabetic model at 4 and 7 days after tooth extraction.CONCLUSION:
These results suggest that delayed wound healing of oral mucosa in diabetes may be associated with decreased expression levels of these regulatory genes which play important roles in controlling epithelial wound closure.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Extracción Dental
/
Factor de Crecimiento Transformador beta
/
Diabetes Mellitus Tipo 2
Tipo de estudio:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
J Craniomaxillofac Surg
Asunto de la revista:
ODONTOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM