Visual detection of filaria-specific IgG4 in urine using red-colored high density latex beads.
Parasitol Int
; 62(1): 32-5, 2013 Feb.
Article
en En
| MEDLINE
| ID: mdl-22982821
ABSTRACT
The use of urine for the immunodiagnosis of lymphatic filariasis has a definite advantage the sample collection is not invasive and thus well accepted by people. Urine-based ELISA to detect filaria-specific IgG4 has been used successfully. However, ELISA requires equipment such as a microplate reader, which is often not available in most endemic areas. We have developed a new visual immunodiagnosis that detects urinary IgG4 using red-colored latex beads (bead test). The sensitivity was 87.2% when ICT antigen test positive people were regarded as the standard (136/156), and the specificity was 97.2% with the non-endemic people in Japan and Bangladesh, and the urine ELISA negatives in Sri Lanka (1264/1300). In a prevalence study, the bead test could detect filarial infection more effectively than ICT test among young children in Sri Lanka, indicating the usefulness of the visual test in epidemiological studies.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Filariasis Linfática
/
Pruebas Inmunológicas
/
Inmunoglobulina G
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Microesferas
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
Límite:
Adolescent
/
Animals
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Child
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Child, preschool
/
Humans
Idioma:
En
Revista:
Parasitol Int
Asunto de la revista:
PARASITOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón