Identification and characterization of a human IL-10 receptor antagonist.
Hum Immunol
; 74(1): 28-31, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-23000375
ABSTRACT
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that works through IL-10 receptor alpha subunit- and suppresses immune responses in many infectious diseases such as leishmaniasis as well as in cancer. Therefore, in order to restore the host-protective immune responses in such diseases, an antagonist to this cytokine is a pressing need. Herein, using phage peptide library display, we have identified a dodecameric peptide that functions as an antagonist to human IL-10 receptor in an IL-10-induced STAT3 phosphorylation assay. The peptide antagonist's ability to restore anti-leishmanial function in CD40-activated macrophages was also tested. We observed that the peptide reduced IL-10-induced STAT-3 phosphorylation and enhanced CD40-activated leishmanial functions in macrophages.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligopéptidos
/
Leishmania donovani
/
Biblioteca de Péptidos
/
Receptores de Interleucina-10
/
Macrófagos
/
Antiprotozoarios
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Hum Immunol
Año:
2013
Tipo del documento:
Article
País de afiliación:
India