Preparation and characterization of monodisperse core-shell Fe3O4@SiO2 microspheres and its application for magnetic separation of nucleic acids from E. coli BL21.
J Biomed Nanotechnol
; 8(6): 1000-5, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-23030008
ABSTRACT
In this article, we present an easy route to prepare monodisperse core-shell Fe3O4@SiO2 microspheres with uniform size and shape. Their structures and properties were studied by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FT-IR), and vibrating sample magnetometer (VSM), respectively. The results showed that spherical Fe3O4 microspheres with well dispersion have a rough surface and an average diameter (about 500 nm). After the modification with silica, the particles have a well-defined core-shell structure and a much smoother surface and larger particle diameter (about 600 nm). Furthermore, VSM measurements indicated that the as-prepared Fe3O4 and Fe3O4@SiO2 microspheres were superparamagnetic at room temperature and the saturation magnetization (M(s)) were 58.110 emu/g and 33.479 emu/g, respectively. And then, the prepared monodisperse core-shell Fe3O4@SiO2 microspheres were subsequently applied to separate nucleic acids from the bacteria (E. coli BL21) and verified the great application prospects for bioseparation technology of the biomoleculars.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácidos Nucleicos
/
Dióxido de Silicio
/
Nanotecnología
/
Escherichia coli
/
Óxido Ferrosoférrico
/
Nanopartículas del Metal
Idioma:
En
Revista:
J Biomed Nanotechnol
Año:
2012
Tipo del documento:
Article