A novel method of diffraction imaging flow cytometry for sizing microspheres.
Opt Express
; 20(20): 22245-51, 2012 Sep 24.
Article
en En
| MEDLINE
| ID: mdl-23037372
ABSTRACT
We report a novel method of diffraction imaging flow cytometry to measure and analyze size distribution of microspheres. An automated and robust image processing software based on the short-time-Fourier-transform algorithm has been developed to analyze the characteristic and spatially varying oscillations of side scatters recorded as a diffraction image. Our results demonstrate that the new method allows accurate and rapid determination of single microspheres' diameters ranging from 1 to 100 µm. The capacity for analysis of light scattering by two-sphere aggregates has been demonstrated but analytical tools for characterization of aggregates by multiple microspheres remain to be developed.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tamaño de la Partícula
/
Reconocimiento de Normas Patrones Automatizadas
/
Inteligencia Artificial
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Interpretación de Imagen Asistida por Computador
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Citometría de Flujo
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Microesferas
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Nefelometría y Turbidimetría
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China