Diversity of viruses detected by deep sequencing in pigs from a common background.
J Vet Diagn Invest
; 24(6): 1177-9, 2012 Nov.
Article
en En
| MEDLINE
| ID: mdl-23051826
ABSTRACT
Although advances in nucleic acid sequencing have enabled the discovery of many infectious agents, challenges remain for scientists and veterinary diagnosticians trying to design animal studies with a minimum of variables and to interpret laboratory results. To evaluate pyrosequencing technology as a potential screening method to estimate the virome in pigs, fecal samples were collected from 4 pigs out of a group of 175 that had been raised together since birth. A number of viruses were detected, demonstrating the application of this technology to determine the background "noise" in the pigs. However, pyrosequencing also demonstrated the diversity of viruses within a group of animals and how that can confound experimental design and obscure a definitive diagnosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Porcinos
/
Variación Genética
/
Virus
/
Virosis
Límite:
Animals
Idioma:
En
Revista:
J Vet Diagn Invest
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos